Recombinant Human DNA repair protein RAD51 homolog 4(RAD51D)

N-terminal GST-tagged

62 kDa

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.

Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.

Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, telomere

Breast-ovarian cancer, familial, 4 (BROVCA4)

Full Length

Tris-based buffer, 50% glycerol