Vergleich

Recombinant Escherichia coli Lactose operon repressor(lacI)

ArtNr CSB-EP365825ENV-1
Hersteller Cusabio
Menge 1 mg
Quantity options 1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Konjugat/Tag SUMO, HIS
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTR EKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSS LALHAPSQIVAAIKSRADQLGASVVVSMVERSGVE ACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAAC TNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLV ALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQI QPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVA NDQ
Citations The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.Spronk C.A., Bonvin A.M., Radha P.K., Melacini G., Boelens R., Kaptein R.Structure 7:1483-1492(1999)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Manufacturer - Targets
lacI
Manufacturer - Conjugate / Tag
N-terminal 6xHis-SUMO-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
54.6 kDa
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
General Research Areas
Microbiology
Relevance
Repressor of the lactose operon. Binds allolactose as an inducer.
Biologically Active
Not Test
Expression Region
1-360aa
Protein Length
Full Length
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Repressor of the lactose operon. Binds allolactose as an inducer.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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