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Recombinant Bacillus subtilis Modification methylase BglII(bglIIM)

ArtNr CSB-EP670450BRI-200
Hersteller Cusabio
Menge 200 ug
Quantity options 1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Konjugat/Tag SUMO, HIS
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MSEDQYKQIKLHLGMEDDNEDLPNHIPSSFPKQHL NKIYNGDTMNMLLDIPDNSVDLVVTSPPYNINKFK NDRRPLEEYLKWQTEIIEQCHRVLKPSGSIFWQVG TYVNDSGAHIPLDIRFFPIFESLGMFPRNRIVWVR PHGLHANKKFAGRHETILWFTKTPEYKFFLDPIRV PQKYANKKHYKGDKKGELSGDPLGKNPGDVWAFRN VRHNHEEDTIHPTQYPEDMIERIVLSTTEPNDIVL DPF
Protein Familie N(4)/N(6)-methyltransferase family, N(4) subfamily
Citations Cloning and characterization of the BglII restriction-modification system reveals a possible evolutionary footprint.Anton B.P., Heiter D.F., Benner J.S., Hess E.J., Greenough L., Moran L.S., Slatko B.E., Brooks J.E.Gene 187:19-27(1997)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias N(4)- cytosine-specific methyltransferase BglII
Lieferbar
Manufacturer - Conjugate / Tag
N-terminal 6xHis-SUMO-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
58 kDa
Relevance
This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease.
Expression Region
1-360aa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease.
Gene Names
bglIIM
Sequence Info
Full Length
Organism
Bacillus subtilis (strain 168)
Storage Buffer
Tris-based buffer, 50% glycerol

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 ug
Lieferbar: In stock
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