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Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE(tsaE)

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE(tsaE)

ArtNr	CSB-MP360227ENV-20
Hersteller	Cusabio
Menge	20 ug
Quantity options	10 ug 100 ug 1 mg 20 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Host	Mammalian cells
Purity	Greater than 90% as determined by SDS-PAGE.
Sequence	MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYG DLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYT LDNLMVYHFDLYRLADPEELEFMGIRDYFANDAIC LVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAV SSAGELLLARLAG
Protein Familie	TsaE family
Citations	The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase. Tsui H.-C.T., Zhao G., Feng G., Leung H.-C.E., Winkler M.E. Mol. Microbiol. 11:189-202(1994)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
Lieferbar
Manufacturer - Conjugate / Tag	C-terminal hFc-tagged
Storage Conditions	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight	42.9 kDa
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Relevance	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Expression Region	1-153aa
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Subcellular Location	Cytoplasm
Gene Names	tsaE
Sequence Info	Full Length
Organism	Escherichia coli (strain K12)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CSB-MP360227ENV-20-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 20 ug

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Lieferung vsl. bis 04.09.2025

Express-Lieferung: 3 Tage sparen

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