Item no. |
Z02190-50 |
Manufacturer |
GenScript
|
Amount |
50 ug |
Quantity options |
1 mg (2x500 ug)
10 ug
50 ug
|
Category |
|
Type |
Proteins |
Specific against |
Human (Homo sapiens) |
Purity |
The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods: (a) RP-HPLC anaylsis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
Sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z02190_50-GM_CSF_Sargramostim_Human,Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript, Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim) , is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26,000-32,000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein.</td></tr><tr><th>M.W.</th><td colspan="7"> 26,000-32,000 Da</td></tr><tr><th>Purity</th><td colspan="7"> The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods: (a) RP-HPLC anaylsis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of sargramostim</td></tr><tr><th>Storage</th><td colspan="7"> Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18C. Upon reconstitution Sargramostim should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.</td></tr><tr><th>Specificity</th><td colspan="7"> The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.</td></tr> |
Similar products |
Granulocyte |
Available |
|
Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0x106 IU/ mg. |
Country of Origin |
USA |
Storage Conditions |
Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18C. Upon reconstitution Sargramostim should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Molecular Weight |
26, 000-32, 000 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Manufacturer - Specificity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0x106 IU/ mg. |
Description |
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript, Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim) , is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 26, 000-32, 000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. |
Reconstitution |
It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Formulation |
The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer. |
Endotoxin Level |
Less than 0.1 ng/ug (1 EU/ug) of sargramostim |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.