Recombinant human PKLR protein

Recombinant Protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Cancer

Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

PKLR, Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK, ATGP0714-10ug, ATGP0714-20ug, ATGP0714-50ug, ATGP0714-100ug, ATGP0714-250ug, ATGP0714-500ug, ATGP0714-1mg, ATGP0714-10, ATGP0714-20, ATGP0714-50, ATGP0714-100, ATGP0714-250, ATGP0714-500, ATGP0714-1