Pro-neuregulin-1, membrane-bound isoform, active (NRG1), Recombinant, Human

Blue Ice

7

Lyophilized from 0.2um filtered PBS, pH 7.4

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. {ECO:0000269|PubMed:1348215, ECO:0000269|PubMed:7902537}.

Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 50ng/ml, corresponding to a specific activity of ＞ 2.0x10e4 IU/mg.

Sequence:
SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q

Molecular Weight:
7.0kD

PubMed ID:
1350381; 7509448; 8096067; 7782315; 14702039; 16421571; 9333014; 12145742; 17565985; 15489334; 7689552; 1348215; 7902537; 10523851; 10597312; 10867024; 8062828; 8639490

Gene Name:
NRG1, GGF, HGL, HRGA, NDF, SMDF

Swiss Prot:
Q02297

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.