TWF1, Recombinant, Human, aa1-252, His-Tag (Twinfilin-1, PTK9, Protein Tyrosine Kinase 9)

Blue Ice

31, 5

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 40% glycerol.

TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of ∼20 residues. The two ADF-H domains are ∼20% homologous to ADF/cofilin and to each other.

Recombinant protein corresponding to aa1-252 from human TWF1, fused to His-tag at N- terminal expressed in E. coli.

Molecular Weight:
~31.5kD (277aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.