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Recombinant Human Serpin F2 (Alpha-2-Antiplasmin) Protein

Recombinant Human Serpin F2 (Alpha-2-Antiplasmin) Protein

ArtNr	230-30171-50
Hersteller	Raybiotech
Menge	50 ug
Quantity options	10 ug 100 ug 200 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Lyophilized
Applikationen	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	Human, HEK293
Konjugat/Tag	HIS
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins\|COVID-19\|Proteins\|Thrombosis Proteins
Manufacturer - Conjugate / Tag	N-terminal his-tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of 50 kDa. Due to the abundant glycosylation, it migrates as approximately 60 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 50 kDa band. See deglycosylation analysis in SDS-PAGE image.
Gene Symbols	SERPINF2
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region	Asn40-Lys491
Protein Name & Synonyms	Serpin Family F Member 2, Alpha-2-Plasmin Inhibitor, Serine (Or Cysteine) Proteinase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, ALPHA-2-PI, Alpha-2-AP, Serpin F2, PLI, AAP, A2AP, API
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by BCA protein assay (lot specific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under denature conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.