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Recombinant MERS S1 Subunit Protein, RBD

Recombinant MERS S1 Subunit Protein, RBD

ArtNr	230-30204-100
Hersteller	Raybiotech
Menge	100 ug
Quantity options	100 ug 500 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Liquid
Applikationen	WB, IP, ELISA, Dot
Specific against	other
Host	Human, HEK293
Konjugat/Tag	HIS
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Viral Proteins
Manufacturer - Conjugate / Tag	C-terminal his-tag
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of ˆ¼26 kDa. Due to the abundant glycosylation, it migrates as approximately ˆ¼37 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one reduced ˆ¼26 kDa band. See deglycosylation analysis image below.
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Expressed Region	Glu367-Tyr606
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial), determined by BCA protein assay (lot specific)
Endotoxin Level	< 0.5 EU per µg of the protein as determined by the LAL method
SDS-PAGE Image	Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼25 kDa), thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.