Recombinant human NEDD8 protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol

Ubiquitination

< 1 EU per 1ug of protein (determined by LAL method)

NEDD8, Neural precursor cell expressed developmentally down-regulated 8, ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, ubiquitin like protein Nedd 8, ubiquitin like protein Nedd8., NED0905-10ug, NED0905-20ug, NED0905-50ug, NED0905-100ug, NED0905-250ug, NED0905-500ug, NED0905-1mg, NED0905-10, NED0905-20, NED0905-50, NED0905-100, NED0905-250, NED0905-500, NED0905-1