Recombinant Human CD47 Protein

< 1.0 EU/μg of the protein by LAL method.

ice pack

14.56 kDa

Recombinant Human CD47 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Pro139) of human CD47 (Accession #NP_001768.1) fused with an 6×His tag at the C-terminus.

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

C-His

Immune Checkpoint, Bio-Markers & CD Antigens, Biosimilar Drug Targets

1. Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 5 μg/mL (100 μL/well) can bind Human SIRPA with a linear range of 0. 488-189. 26 ng/mL.|Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (Catalog: RP01306) at 2 μg/mL (100 μL/well) can bind Human SIRP-alpha/CD172a (Catalog: RP00171) with a linear range of 0. 06-22. 3 ng/mL.|3. Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(Catalog: RP00171) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (Catalog: RP01306) with an affinity constant of 11. 1 nM as determined in BLI assay (Gator).

14.56 kDa