Human AKI Array C1 (4)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-AKI-1-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Acute Kidney Injury Antibody Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
20
Target Names
TIM-1 (KIM-1), Albumin, Osteopontin (SPP1), TFF3 (Trefoil Factor 3), Beta-2 Microglobulin, Clusterin, CXCL16, Osteoactivin (GPNMB), FABP1, MCP-1 (CCL2), TNF RI (TNFRSF1A), Calbindin, IP-10 (CXCL10), Cystatin C, HGF, MIF, Lipocalin-2 (NGAL), TIMP-1, VCAM-1 (CD106), VEGF-A
Targets Detected
Albumin, Beta-2 Microglobulin, Calbindin, CCL2, CD106, Clusterin, CXCL10, CXCL16, Cystatin C, FABP1, GPNMB, HGF, IP-10, KIM-1, Lipocalin-2, MCP-1, MIF, NGAL, Osteoactivin, Osteopontin, SPP1, TFF3, TIM-1, TIMP-1, TNF RI, Trefoil Factor 3, VCAM-1, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Acute Kidney Injury (AKI) Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
Chen T., et al., Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Scho¨ nlein Purpura Patients with Renal Involvement. PLOS One. Published: June 25, 2014 DOI: 10.1371/journal.pone.0101026
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 667,40 €
Preis: 667,40 €
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