Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Assay |
Specific against |
Human |
Menge |
4 Sample Kit |
ArtNr |
AAH-AKI-1-4 |
Targets |
CLU ;;CALB1;;CCL2;;CST3;;CXCL10;;FABP1;;HAVCR1;;IFNG;;LCN2;;TIMP1;;VCAM1;;VEGFA |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Short description |
RayBio C-Series Human Acute Kidney Injury Antibody Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types. |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Number of Targets Detected |
20 |
Target Names |
TIM-1 (KIM-1), Albumin, Osteopontin (SPP1), TFF3 (Trefoil Factor 3), Beta-2 Microglobulin, Clusterin, CXCL16, Osteoactivin (GPNMB), FABP1, MCP-1 (CCL2), TNF RI (TNFRSF1A), Calbindin, IP-10 (CXCL10), Cystatin C, HGF, MIF, Lipocalin-2 (NGAL), TIMP-1, VCAM-1 (CD106), VEGF-A |
Targets Detected |
Albumin, Beta-2 Microglobulin, Calbindin, CCL2, CD106, Clusterin, CXCL10, CXCL16, Cystatin C, FABP1, GPNMB, HGF, IP-10, KIM-1, Lipocalin-2, MCP-1, MIF, NGAL, Osteoactivin, Osteopontin, SPP1, TFF3, TIM-1, TIMP-1, TNF RI, Trefoil Factor 3, VCAM-1, VEGF-A |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
Kit Components |
- Human Acute Kidney Injury (AKI) Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Membrane |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Chemiluminescence |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
Chen T., et al., Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Scho¨ nlein Purpura Patients with Renal Involvement. PLOS One. Published: June 25, 2014 DOI: 10.1371/journal.pone.0101026 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.