Human Apoptosis Array G1 (4)

Hersteller Raybiotech
Typ Assay
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-APO-G1-4
eClass 6.1 32161000
eClass 9.0 32161000
Short description
RayBio G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Secondary categories
Products///Functional Assays///Apoptosis Assays
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
Target Names
bad, bax, bcl-2, bcl-w, BID, BIM, Caspase-3, Caspase-8, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), cIAP-2, Cytochrome C, DR6 (TNFRSF21), Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), HSP27, HSP60, HSP70, HTRA2, IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1 R, livin, p21, p27, p53, SMAC, Survivin (BIRC5), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNF alpha, TNF beta (TNFSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), XIAP
Targets Detected
4-1BB, Apo-1, bad, bax, bcl-2, bcl-w, BID, BIM, BIRC5, Caspase-3, Caspase-8, CD40, CD40 Ligand, cIAP-2, Cytochrome C, DR4, DR5, DR6, Fas, Fas Ligand, HSP27, HSP60, HSP70, HTRA, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, Livin, p21, p27, p53, SMAC, Survivin, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10A, TNFRSF10B, TNFRSF10D, TNFRSF21, TNFRSF5, TNFRSF6, TNFRSF9, TNFSF1B, TNFSF5, TNFSF6, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, XIAP
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Apoptosis Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Result Output
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
1. The Presence of HIV-1 Tat Second Exon Delays Fas-Mediated Apoptosis in CD4+ T lymphocytes: a Potential Mechanism for Persistent Viral Production
2. Ibrahim M., et al. alpha-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-kappaB and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27, 8:1629-47. doi: 10.2147/DDDT.S66105
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 584,44 €
Preis: 584,44 €


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