|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Assay |
|
Specific against |
Human |
|
Menge |
8 Sample Kit |
|
ArtNr |
AAH-AKI-1-8 |
|
eClass 6.1 |
32161000 |
|
eClass 9.0 |
32161000 |
|
Lieferbar |
 |
| Short description |
RayBio C-Series Human Acute Kidney Injury Antibody Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types. |
| Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
| Number of Targets Detected |
20 |
| Target Names |
TIM-1 (KIM-1), Albumin, Osteopontin (SPP1), TFF3 (Trefoil Factor 3), Beta-2 Microglobulin, Clusterin, CXCL16, Osteoactivin (GPNMB), FABP1, MCP-1 (CCL2), TNF RI (TNFRSF1A), Calbindin, IP-10 (CXCL10), Cystatin C, HGF, MIF, Lipocalin-2 (NGAL), TIMP-1, VCAM-1 (CD106), VEGF-A |
| Targets Detected |
Albumin, Beta-2 Microglobulin, Calbindin, CCL2, CD106, Clusterin, CXCL10, CXCL16, Cystatin C, FABP1, GPNMB, HGF, IP-10, KIM-1, Lipocalin-2, MCP-1, MIF, NGAL, Osteoactivin, Osteopontin, SPP1, TFF3, TIM-1, TIMP-1, TNF RI, Trefoil Factor 3, VCAM-1, VEGF-A |
| Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
| Kit Components |
- Human Acute Kidney Injury (AKI) Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
| Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
| Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
| Storage |
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months. |
| Solid Support |
Membrane |
| Design Principle |
Sandwich-based |
| Result Output |
Semi-Quantitative |
| Detection Method |
Chemiluminescence |
| Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Citation |
Chen T., et al., Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Scho¨ nlein Purpura Patients with Renal Involvement. PLOS One. Published: June 25, 2014 DOI: 10.1371/journal.pone.0101026 |
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