Human Angiogenesis Array C1000 (4)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-ANG-1000-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array C1 & C2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
43
Target Names
Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, bFGF, EGF, ENA-78 (CXCL5), Endostatin, GCSF, GM-CSF, GRO alpha/beta/gamma, I-309 (TCA-3/CCL1), IFN-gamma, IGF-1, IL-10, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-8 (CXCL8), I-TAC (CXCL11), Leptin, MCP-1 (CCL2), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PDGF-BB, PECAM-1 (CD31), PLGF, RANTES (CCL5), TGF beta 1, Thrombopoietin (TPO), Tie-2, TIMP-1, TIMP-2, TNF alpha, uPAR, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected
Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, bFGF, CCL1, CCL13, CCL2, CCL5, CCL7, CD31, CXCL11, CXCL5, CXCL8, EGF, ENA-78, Endostatin, G-CSF, GM-CSF, GRO, I-309, I-TAC, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-2, IL-4, IL-6, IL-8, Leptin, MARC, MCP-1, MCP-3, MCP-4, MMP-1, MMP-9, PDGF-BB, PECAM-1, PlGF, RANTES, TCA-3, TGF beta 1, Thrombopoietin, Tie-2, TIMP-1, TIMP-2, TNF alpha, TPO, uPAR, VEGF-A, VEGF-D, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006, 17:279-290.
2. Konig J., et al. Placental mesenchymal stromal cells derived from blood vessels or avascular tissues: What is the better choice to support endothelial cell function?. Stem Cells and Development, Sept 2014 epub ahead of print. doi:10.1089/scd.2014.0115)

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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 705,82 €
Preis: 705,82 €
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