Human Chemokine Array C1

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

BLC (CXCL13), CCL28 (MEC), Ck beta 8-1 (CCL23), CTACK (CCL27), CXCL16, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fractalkine (CX3CL1), GCP-2 (CXCL6), GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), I-309 (TCA-3/CCL1), I-TAC (CXCL11), IL-8 (CXCL8), IP-10 (CXCL10), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), NAP-2 (PPBP/CXCL7), PARC (CCL18), RANTES (CCL5), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), TARC (CCL17), TECK (CCL25)

Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.

Sandwich-based

Chemiluminescence

1. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011, 186:453–463.
2. De Boeck A., et al. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression. Proteomics. 2013 Jan, 13(2):379-88. doi: 10.1002/pmic.201200179. Epub 2013 Jan 6.
3. Farahi N, Cowburn A S, Upton P D, Deighton J, Sobolewski A, et al.Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells. J Immunol. 2007, 179:1264–1273
4. Sze S, Kleijn D, Lai R, Tan E, Zhao H, et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693
5. Xu Y, Ganem D. Induction of chemokine production by latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells. J Gen Virol. 2007, 88:45-50.
6. Durand S H, Flacher V, Romeas A, Carrouel F, Colomb E, et al.Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts.J Immunol, 2006, 176: 2880-2887
7. Bingera T, Sticha S, Andreasa K, Kapsb C, Sezerc O, et al. Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25. Exp Cell Res. 2009, 315:1468-1479
8. Hsu M, Wu S, Chang S, Su I, Tsai C, et al.Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells. J. Virol. April 2008 vol. 82 no. 7 3679-3688
9. Rizzo M., et al. Low Molecular Weight Hyaluronan-Pulsed Human Dendritic Cells Showed Increased Migration Capacity and Induced Resistance to Tumor Chemoattraction. PLOS One, Published: September 19, 2014. DOI: 10.1371/journal.pone.0107944