Human Chemokine Array C1 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CHE-1-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
38
Target Names
BLC (CXCL13), CCL28 (MEC), Ck beta 8-1 (CCL23), CTACK (CCL27), CXCL16, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fractalkine (CX3CL1), GCP-2 (CXCL6), GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), I-309 (TCA-3/CCL1), I-TAC (CXCL11), IL-8 (CXCL8), IP-10 (CXCL10), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), NAP-2 (PPBP/CXCL7), PARC (CCL18), RANTES (CCL5), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), TARC (CCL17), TECK (CCL25)
Targets Detected
BLC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL16, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, Fractalkine, GCP-2, GRO, GRO alpha, HCC-4, I-309, I-TAC, IL-8, IP-10, Lymphotactin, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MPIF-1, MPIF-2, NAP-2, PARC, PPBP, RANTES, SDF-1 alpha, SDF-1 beta, TARC, TCA-3, TCK-1, TECK, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011, 186:453–463.
2. De Boeck A., et al. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression. Proteomics. 2013 Jan, 13(2):379-88. doi: 10.1002/pmic.201200179. Epub 2013 Jan 6.
3. Farahi N, Cowburn A S, Upton P D, Deighton J, Sobolewski A, et al.Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells. J Immunol. 2007, 179:1264–1273
4. Sze S, Kleijn D, Lai R, Tan E, Zhao H, et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693
5. Xu Y, Ganem D. Induction of chemokine production by latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells. J Gen Virol. 2007, 88:45-50.
6. Durand S H, Flacher V, Romeas A, Carrouel F, Colomb E, et al.Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts.J Immunol, 2006, 176: 2880-2887
7. Bingera T, Sticha S, Andreasa K, Kapsb C, Sezerc O, et al. Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25. Exp Cell Res. 2009, 315:1468-1479
8. Hsu M, Wu S, Chang S, Su I, Tsai C, et al.Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells. J. Virol. April 2008 vol. 82 no. 7 3679-3688
9. Rizzo M., et al. Low Molecular Weight Hyaluronan-Pulsed Human Dendritic Cells Showed Increased Migration Capacity and Induced Resistance to Tumor Chemoattraction. PLOS One, Published: September 19, 2014. DOI: 10.1371/journal.pone.0107944
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 542,97 €
Preis: 542,97 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen