Human Cytokine Array C8

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Hersteller Raybiotech
Typ Array
Specific against Human
Menge 2 Sample Kit
ArtNr AAH-CYT-8-2
eClass 6.1 32161000
eClass 9.0 32161000
Short description
RayBio C-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
Target Names
Activin A, ALCAM (CD166), CD80 (B7-1), BMP-5, BMP-7, Cardiotrophin-1 (CT-1), CD14, CXCL16, DR6 (TNFRSF21), Endoglin (CD105), ErbB3, E-Selectin, Fas Ligand (TNFSF6), ICAM-2 (CD102), IGF-2, IL-1 R2, IL-10 R beta, IL-13 R alpha 2, IL-18 BP alpha, IL-18 R beta (AcPL), MMP-3, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-21 R, IL-5 R alpha, IL-9, IP-10 (CXCL10), LAP/TGF beta 1, Leptin R, LIF, L-Selectin (CD62L), M-CSF R, MMP-1, MMP-13, MMP-9, MPIF-1 (CCL23), NGFR (TNFRSF16), PDGF-AA, PDGF-AB, PDGF R alpha, PDGF R beta, PECAM-1 (CD31), Prolactin, SCF R (CD117/c-kit), SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), TGF alpha, TGF beta 2, Tie-1, Tie-2, TIMP-4, VE-Cadherin (CDH5), VEGFR2, VEGFR3
Targets Detected
AcPL, Activin A, ALCAM, B7-1, BMP-5, BMP-7, c-kit, Cardiotrophin-1, CCL23, CD102, CD117, CD122, CD14, CD170, CD31, CD62L, CD80, CDH5, Common gamma Chain, CT-1, CXCL10, CXCL12 beta, CXCL16, DR6, E-Selectin, Endoglin, ErbB3, Fas Ligand, ICAM-2, IGF-2, IL-1 R2, IL-10 R beta, IL-13 R alpha 2, IL-18 BP alpha, IL-18 R beta, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-5 R alpha, IL-9, IP-10, L-Selectin, LAP/TGF beta 1, Leptin R, LIF, M-CSF R, MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1, NGFR, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PECAM-1, Prolactin, SCF R, SDF-1 beta, Siglec-5, TGF alpha, TGF beta 2, Tie-1, Tie-2, TIMP-4, TNFRSF16, TNFRSF21, TNFSF6, VE-Cadherin, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C8 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Design Principle
Result Output
Detection Method
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
1. Yang CC, Shih YH, Ko MH, Hsu SY, Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008, 3(10):e3336. doi:10.1371/journal.pone.0003336
2. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis
3. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013, 73:662–671.
4. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007, 67(8):3646–3653.
5. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007, 2(9): e941. doi:10.1371/journal.pone.0000941
6. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M, et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008, 93: 2255–2262
7. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006, 26(5):483-490.
8. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008, 8:e18.
9. Le Bras G., et al. TGFbeta loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion. Experimental Cell Research Available online 23 July 2014. DOI: 10.1016/j.yexcr.2014.07.021
10. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Published Online First August 4, 2014, doi: 10.1158/1541-7786.MCR-14-0159

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Menge: 2 Sample Kit
Lieferbar: In stock


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