Human Cytokine Array G2000 (8)

Hersteller Raybiotech
Typ Array
Specific against Human
Menge 8 Sample Kit
ArtNr AAH-CYT-G2000-8
eClass 6.1 32161000
eClass 9.0 32161000
Short description
RayBio G-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array G6, G7, & G8. Detects 174 Human Cytokines. Suitable for all liquid sample types.
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
Target Names
Adiponectin (ACRP30), Activin A, AgRP, ALCAM (CD166), Amphiregulin, Angiogenin, Angiopoietin-2, Axl, CD80 (B7-1), BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-6, BMP-7, beta-NGF, Betacellulin (BTC), Cardiotrophin-1 (CT-1), CCL28 (MEC), CD14, Ck beta 8-1 (CCL23), CNTF, CTACK (CCL27), CXCL16, DR6 (TNFRSF21), Dtk, EGF, EGFR, ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas Ligand (TNFSF6), Fas Ligand (TNFSF6), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, IL-1 R2, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15, IL-16, IL-17A, IL-18 BP alpha, IL-18 R beta (AcPL), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-2 R alpha, IL-21 R, IL-3, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1, Leptin, Leptin R, LIF, Light (TNFSF14), L-Selectin (CD62L), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-AA, PDGF R alpha, PDGF R beta, PDGF-AB, PDGF-BB, PECAM-1 (CD31), PLGF, Prolactin, RANTES (CCL5), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), gp130, Siglec-5 (CD170), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 2, TGF alpha, TGF beta 3, TGF beta 1, Thrombopoietin (TPO), Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VE-Cadherin (CDH5), VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected
AcPL, ACRP30, Activin A, Adiponectin, AgRP, ALCAM, Amphiregulin, Angiogenin, Angiopoietin-2, Apo-1, AXL, B7-1, BDNF, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-5, BMP-6, BMP-7, BTC, c-kit, Cardiotrophin-1, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD102, CD105, CD117, CD122, CD14, CD170, CD31, CD50, CD54, CD62L, CD80, CK beta 8-1, CNTF, Common gamma Chain, CT-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL16, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, DR6, Dtk, E-Selectin, EGF, EGFR, ENA-78, Endoglin, Eotaxin-1, Eotaxin-2, Eotaxin-3, ErbB1, ErbB3, Fas, Fas Ligand, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, HCC-4, HGF, I-309, I-TAC, ICAM-1, ICAM-2, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R2, IL-1 R4, IL-1 Ra, IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15, IL-16, IL-17, IL-18 BP alpha, IL-18 R beta, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-3, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8, IL-9, IP-10, KGF, L-Selectin, LAP/TGF beta 1, Leptin, Leptin R, LIF, LIGHT, Lymphotactin, M-CSF, M-CSF R, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1, MPIF-2, MSP alpha/beta, NAP-2, NGFR, NT-3, NT-4, Oncostatin M, Osteoprotegerin, PARC, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PlGF, PPBP, Prolactin, RANTES, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Siglec-5, ST2, TARC, TCA-3, TECK, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10D, TNFRSF11B, TNFRSF16, TNFRSF18, TNFRSF21, TNFRSF6, TNFSF14, TNFSF18, TNFSF1B, TNFSF6, TPO, TRAIL R3, TRAIL R4, uPAR, VE-Cadherin, VEGF-A, VEGF-D, VEGFR2, VEGFR3, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G2000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Result Output
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
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2. Brunt KR., et al. Ex Vivo Akt/HO-1 Gene Therapy to Human Endothelial Progenitor Cells Enhances Myocardial Infarction Recovery. Cell Transplant. 2012, 21(7):1443-61.
3. Duncan JA, Gao X, Huang MT-H, O'Connor BP, Thomas CE, et al. Neisseria gonorrhoeae Activates the Proteinase Cathepsin B to Mediate the Signaling Activities of the NLRP3 and ASC-Containing Inflammasome. J Immunol. 2009, 182:6460-6469.
4. Ye Z, Lich JD, Moore CB, Duncan JA, Williams KL, Ting JP-Y. ATP Binding by Monarch-1/NLRP12 Is Critical for Its Inhibitory Function. Mol Cell Biol. 2008, 28:1841–1850.
5. Kocaoemer A, Kern S, Kluter H, Bieback K, Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue. Stem Cell. 2007, 25:1270–1278.
6. Nolting T., et al., Measurement of soluble inflammatory mediators in cerebrospinal fluid of human immunodeficiency virus–positive patients at distinct stages of infection by solid-phase protein array. Journal of NeuroVirology, 15: 390–400, 2009
7. Vasish, Seewoobudul, Shaojia Lua, Hongjun Penga, and Lifeng Wanga. "Elevated Specific Peripheral Cytokines Found in Major Depressive Disorder Patients with Childhood Trauma Exposure: A Cytokine Antibody Array Analysis." Comprehensive Psychiatry. ScienceDirect, Oct. 2013. Web.
8. Tape C., Norrie I., Worboys J., Lim L., Lauffenburger D., Jorgensen C. Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture. Molecular and Cellular Proteomics (2014)
9. Park JE., Hypoxic Tumor Cell Modulates Its Microenvironment to Enhance Angiogenic and Metastatic Potential by Secretion of Proteins and Exosomes. Molecular & Cellular Proteomics, June 1, 9, 1085-1099.
10. Eiro N., et al. Potential therapeutic effect of the secretome from human uterine cervical stem cells against both cancer and stromal cells compared with adipose tissue stem cells. Oncotarget, Sept 2014 epub ahead of print.
11. Fujita K, Ewing C M , Sokoll L J, Elliott D J, Cunningham M, et al.Cytokine Profiling of Prostatic Fluid From Cancerous Prostate Glands Identifies Cytokines Associated With Extent of Tumor and Inflammation. Prostate. 2008, 68:872–882.

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Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 3.636,58 €
Preis: 3.636,58 €


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