Human Cytokine Array G3 (8)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 8 Sample Kit
ArtNr AAH-CYT-G3-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
42
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin
Targets Detected
Angiogenin, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CXCL1, CXCL12 alpha, CXCL8, CXCL9, EGF, ENA-78, G-CSF, GM-CSF, GRO, GRO alpha, I-309, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, Leptin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MDC, MIG, MIP-1 delta, Oncostatin M, PDGF-BB, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TGF beta 1, Thrombopoietin, TNF alpha, TNF beta, TNFSF1B, TPO, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
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2. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007, 43:1117–1124.
3. Wang S, Yang J, Qian J, Wezeman M, Kwak LW, Yi Q. Tumor evasion of the immune system: inhibiting p38 MAPK signaling restores the function of dendritic cells in multiple myeloma. Blood. 2006, 107:2431-2439.
4. Eldeen M B, Deshmane S L, Simbiri K, Khalili K, Amini S, et al.MH2 domain of Smad3 reduces HIV-1 Tat-induction of cytokine secretion. J Neuroimmunol. 2006, 176:174 – 180
5. Gerrits J H, Wetering J, Weimar W, Besouw N M. Donor-Reactive Cytokine Production After HLA-Identical Living Related Kidney Transplantation: A Protein-Array Analysis.Transplantation Proceedings, 2006, 38, 2825–2827
6. Rethi B, Gogolak P, Szatmari I, Veres A, et al. SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte-derived dendritic cells. Blood. 2006, 107:2821-2829.
7. Li J, Bentsman G, Potash M, Volsky D.Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection.BMC Neuroscience.2007, 8:31
8. Fiorucci M, Boulant S, Fournillier A, Abraham J, Lavergne J, et al.Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries.J Gen Virol. 2007, 88:1149–1162
9. Potian JA, Aviv H, Ponzio NM, Harrison JS, Rameshwar P. Veto-Like Activity of Mesenchymal Stem Cells: Functional Discrimination Between Cellular Responses to Alloantigens and Recall Antigens. J Immunol. 2003, 17(1)3426.3434.
10. Julia P, Daniel S.Effect of gonococcal lipooligosaccharide variation on human monocytic cytokine profile.BMC Microbiology. 2007, 7:7
11. Abbott DW, Yang Y, Hutti JE, Madhavarapu S, Kelliher MA, Cantley LC. Coordinated Regulation of Toll-Like Receptor and NOD2 Signaling by K63-Linked Polyubiquitin Chains. Mol Cell Biol. 2007, 27(17):6012-6025.
12. Benitez S, Camacho M, Bancells C, Vila L, Sanchez-Quesada JL, Ordonez-Llanos J. Wide proinflammatory effect of electronegative low-density lipoprotein on human endothelial cells assayed by a protein array. Biochim Biophys Acta. 2006, 1761:1014-1021.
13. Patrone JB, Bish SE, Stein DC. TNF-alpha-Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response. J Immunol. 2006, 177:1314-1322.
14. Gogolak P, Rethi B, Szatmari I, Lanyi A, et al. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR-gamma. Blood. 2007, 109:643-652.
15. Chiu TT, Leung WY, Moyer MP, Strieter RM, Rozengurt E. Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kB. Am J Physiol Cell Physiol.2007, 292:C767–C777.
16. Robson RL, Reed NA, Horvat RT. Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties. BMC Infect Dis. 2006, 6:71. Available at http://www.biomedcentral.com/1471-2334/6/71. Last accessed Jan 25, 2008.
17. Bogdanski D, Esenwein SA, Prymak O, Epple M, Muhr G, Koller M. Release of Leucocyte Mediators after Contact to Coated and Non-coated NITI-SMA. Biomedizinische Technik Jg. 2004, 49:562-563.
18. Ritter M, Mennerich D, Weith A, Seither P. Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm. 2005, 2:16. Available at: http://www.journal-inflammation.com/content/2/1/16. Last accessed Jan 25, 2008.
19. Tay SS, McCormack A, Rose ML. Effect of Cognate Human CD4+ T Cell and Endothelial Cell Interactions Upon Chemokine Production. Transplant. 2004, 78(7):987-994.
20. Duenas AI, Aceves M, Orduna A, Diaz R, Crespo MS, Garcia-Rodriguez C. Francisella tularensis LPS induces the production of cytokines in human monocytes and signals via Toll-like receptor 4 with much lower potency than E. coli LPS. Intl Immunol. 2006, 18(5):785-795.
21. Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006, 367:668-678.
22. Kaplin AI, Deshpande DM, Scott E, Krishnan C, Carmen JS, et al. IL-6 induces regionally selective spinal cord injury in patients with the neuroinflammatory disorder transverse myelitis. J Clin Invest. 2005, 115:2731-2741.
23. Nichols MT, Gidey E, Matzakos T, Dahl R, et al. Secretion of Cytokines and Growth Factors Into Autosomal Dominant Polycystic Kidney Disease Liver Cyst Fluid. Hepatology. 2004, 40:836-846.
24. Coppinger JA, O'Connor R, Wynne K, Flanagan M, Sullivan M, et al. Moderation of the platelet releasate response by aspirin. Blood. 2007, 109:4786-4792.
25. Bailey L, Kuroyanagi Y, Franco-Penteado CF, Conran N, Costa FF, et al. Expression of the gamma-globulin gene is sustained by the cAMP-dependent pathway in beta-thalassaemia. Br J Haematol. 2007, 138:382–395.
26. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007, 43:1117–1124.
27. Patrone J, Bish S, Stein D. TNF-alpha -Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response.J Immunol. 2006, 177:1314–1322.
28. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005, 16:256–265.
Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 993,93 €
Preis: 993,93 €
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