Human Cytokine Array G6 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-G6-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
60
Target Names
Angiogenin, BDNF, BLC (CXCL13), BMP-4, BMP-6, Ck beta 8-1 (CCL23), CNTF, EGF, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-6, FGF-7 (KGF), Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, GM-CSF, I-309 (TCA-3/CCL1), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light (TNFSF14), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, PARC (CCL18), PDGF-BB, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TGF beta 3, TNF alpha, TNF beta (TNFSF1B)
Targets Detected
Angiogenin, BDNF, BLC, BMP-4, BMP-6, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL23, CCL24, CCL26, CCL5, CCL7, CCL8, CK beta 8-1, CNTF, CX3CL1, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL9, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 Ra, IL-10, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KGF, Leptin, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIG, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, PARC, PDGF-BB, PPBP, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TNFSF14, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G6 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Brunt KR., et al. Ex Vivo Akt/HO-1 Gene Therapy to Human Endothelial Progenitor Cells Enhances Myocardial Infarction Recovery. Cell Transplant. 2012, 21(7):1443-61.
2. Brunt KR., et al. Ex Vivo Akt/HO-1 Gene Therapy to Human Endothelial Progenitor Cells Enhances Myocardial Infarction Recovery. Cell Transplant. 2012, 21(7):1443-61.
3. Duncan JA, Gao X, Huang MT-H, O'Connor BP, Thomas CE, et al. Neisseria gonorrhoeae Activates the Proteinase Cathepsin B to Mediate the Signaling Activities of the NLRP3 and ASC-Containing Inflammasome. J Immunol. 2009, 182:6460-6469.
4. Ye Z, Lich JD, Moore CB, Duncan JA, Williams KL, Ting JP-Y. ATP Binding by Monarch-1/NLRP12 Is Critical for Its Inhibitory Function. Mol Cell Biol. 2008, 28:1841–1850.
5. Kocaoemer A, Kern S, Kluter H, Bieback K, Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue. Stem Cell. 2007, 25:1270–1278.
6. Mahadevan D., et al. Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3, and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival, and Migration. Hum Genomics Proteomics. 2009 Jun 24, 2009:453634. doi: 10.4061/2009/453634.
7. Nolting T., et al. Measurement of soluble inflammatory mediators in cerebrospinal fluid of human immunodeficiency virus–positive patients at distinct stages of infection by solid-phase protein array. J Neurovirol. 2009 Sep, 15(5-6):390-400. doi: 10.3109/13550280903350192.
8. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.
9. Yang C.Q., MCP-1 Stimulates MMP-9 Expression via ERK 1/2 and p38 MAPK Signaling Pathways in Human Aortic Smooth Muscle Cells. Cell Physiol Biochem 2014, 34:266-276 DOI: 10.1159/000362997
10. Park JE., Hypoxic Tumor Cell Modulates Its Microenvironment to Enhance Angiogenic and Metastatic Potential by Secretion of Proteins and Exosomes. Molecular & Cellular Proteomics, June 1, 9, 1085-1099.
11. Meltzer K, Standley P.Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion.J Am Osteopath Assoc. 2007, 107:527–536
12. Bhattacharyya S, Borthakur A, P Dudeja, Tobacman J. Carrageenan Reduces Bone Morphogenetic Protein-4 (BMP4) and Activates the Wnt/beta-Catenin Pathway in Normal Human Colonocytes.Dig Dis Sci, 2007, 52:2766–2774
13. He T, Peterson TE, Katusic ZS. Paracrine mitogenic effect of human endothelial progenitor cells: role of interleukin-8. Am J Physiol Heart Circ Physiol. 2005, 289:H968-H972.
14. Hausman GJ, Poulos SP, Richardson RL, Barb CR, et al. Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells. J Animal Sci. 2006, 84(7):1666–1681.
15. Nordstrom T, Jendholm J, Samuelsson M, Forsgren A, Riesback K. The IgD-binding domain of the Morazella IgD-binding protein MID (MID 962-1200) activates human B cells in the presence of T cell cytokines. J Leuk Biol. 2006, 79:319–329.

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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 704,05 €
Preis: 704,05 €
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