Human Inflammation Array G3 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-INF-G3-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Inflammation Antibody Array 3 Kit. Detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
40
Target Names
Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), GCSF, GM-CSF, ICAM-1 (CD54), IFN-gamma, I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, IP-10 (CXCL10), MCP-1 (CCL2), MCP-2 (CCL8), M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), PDGF-BB, TIMP-2
Targets Detected
CCL1, CCL11, CCL15, CCL2, CCL24, CCL3, CCL4, CCL5, CCL8, CD54, CXCL10, CXCL8, CXCL9, Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, IL-2, IL-3, IL-4, IL-6, IL-6 R, IL-7, IL-8, IP-10, M-CSF, MCP-1, MCP-2, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MPIF-2, PDGF-BB, RANTES, TCA-3, TGF beta 1, TIMP-2, TNF alpha, TNF beta, TNF RI, TNF RII, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Inflammation Antibody Array G3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. van Rossum A.P., et al. Granulocytosis and thrombocytosis in renal cell carcinoma: a pro-inflammatory cytokine response originating in the tumour. Neth J Med. 2009 May, 67(5):191-4.
2. Ide T, Hirase T, Hazuku A, Ikeda Y, Node K. Angiotensin II Increases Expression of IP-10 and the Renin-Angiotensin System in Endothelial Cells. Hypertens Res. 2008, 31:1257–1267. doi:10.1291/hypres.31.1257
3. Said N, Najwer I, Socha M, Fulton D, Mok S, et al.SPARC Inhibits LPA-Mediated Mesothelial–Ovarian Cancer Cell Crosstalk.Neoplasia.2007, 9:23–35
4. Long D, Blake S, Song X, Lark M, Loeser R. Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13. Arthritis Res Ther. 2008, 10:R23 doi:10.1186/ar2376
5. Miyoshi N, Oubrahim H, Chock PB, Stadtman ER. Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis. PNAS. 2006, 103(6):1727–1731.
6. Fukuda EY, Lad SP, Mikolon DP, Iacobelli-Martinez M, Li E. Activation of Lipid Metabolism Contributes to Interleukin-8 Production during Chlamydia trachomatis Infection of Cervical Epithelial Cells. Infect Immunity. 2005, 73(7):4017-4024.
7. Trinidad AG, de la Puerta ML, Fernandez N, Bayon Y, Crespo MS, Alonso A. Coupling of C3bi to IgG inhibits the tyrosine phosphorylation signaling cascade downstream Syk and reduces cytokine induction in monocytes. J Leukoc.Biol. 2006, 79:1073-1082.
8. Shiraki Y, Ishibashi Y, Hiruma M, Nishikawa A, Ikeda S. Cytokine secretion profiles of human keratinocytes during Thrichophyton tonsurans and Arthroderma benhamiae infections. J Med Microbiol 2006, 55: 1175-1185.
9. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011, 186:453–463.
10. Said NA, Najwer I, Socha MJ, Fulton DJ, Mok SC, Motamed K. SPARC Inhibits LPA-Mediated Mesothelial-Ovarian Cancer Cell Crosstalk. Neoplasia. 2007, 9(1):23–35.
11. Cebalo L, Markotic A. Chemokine Production Predominates in Human Monocytes Infected with Tula Virus. Viral Immunol. 2007, 20:206–213.
12. Matsunaga K, Yanagisawa S, Ichikawa T, Akamatsu K, Koarai A, et al. Molecular Mechanism of the Additive Effects of Leukotriene Modifier in Asthmatic Patients Receiving Steroid Therapy. Allergol Int. 2009, 58:89–96.
13. Matsunaga K, Ichikawa T, Yanagisawa S, Akamatsu K, Koarai A, et al.Clinical Application of Exhaled Breath Condensate Analysis in Asthma: Prediction of FEV 1 Improvement by Steroid Therapy. Respiration. 2009, 78:393–398.
14. Ichikawa T, Matsunaga K, Minakata Y, Yanagisawa S, Ueshima K , et al.Possible Impact of Salivary Infl uence on Cytokine Analysis in Exhaled Breath Condensate. Anal Chem Insights. 2007:2 85–92
15. Shoji J, Inada N, Sawa M. Antibody Array-Generated Cytokine Profile of Tears of Patients with Vernal Keratoconjunctivitis or Giant Papillary Conjunctivitis. Jpn J Ophthalmol. 2006, 50:195-204.
16. Rowley A, Baker S, Shulman S, Garcia F, Guzman-Cottrill J, Detection of Antigen in Bronchial Epithelium and Macrophages in Acute Kawasaki Disease by Use of Synthetic Antibody. J Infect Dis. 2004, 190:856-865.

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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 584,44 €
Preis: 584,44 €
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