Human MMP Array C1 (8)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 8 Sample Kit
ArtNr AAH-MMP-1-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
10
Target Names
MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Targets Detected
MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-8, MMP-9, TIMP-1, TIMP-2, TIMP-4
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Regenerative Potential of Decellularized Porcine Nucleus Pulposus Hydrogel Scaffolds: Stem Cell Differentiation, Matrix Remodeling, and Biocompatibility Studies
2. Kanangat S, Postlethwaite A, Hasty K, Kang A, et al. Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureau: Implications in the pathogenesis of septic arthritis and other soft tissue infections. Arthritis Res Ther. 2006, 8:R176. Available at http://arthritis-research.com/content/8/6/R176. Last accessed January 17, 2008.
3. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression
4. Lisboa RA, Andrade MV, Cunha-Melo. Toll-like receptor activation and mechanical force stimulation promote the secretion of matrix metalloproteinases 1, 3 and 10 of human periodontal fibroblasts via p38, JNK and NF-kB. Arch Oral Biol. 2013, 58(6):731–739.
5. Thielitz A, Vetter R, Schultze B, Wrenger S, Simeoni L, et al.Inhibitors of Dipeptidyl Peptidase IV-Like Activity Mediate Antifibrotic Effects in Normal and Keloid-Derived Skin Fibroblasts. J Investig Dermatol. 2008, 128:855–866
6. Vesey DA, Suen JY, Seow V, et al. PAR2-induced inflammatory responses in human kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2013 Mar 15, 304(6):F737-F750.
7. PAR2-induced inflammatory responses in human kidney tubular epithelial cells
8. Yang B., et al. Chrysin inhibits metastatic potential of human triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. J Appl Toxicol. 2014 Jan, 34(1):105-12. doi: 10.1002/jat.2941. Epub 2013 Oct 10
9. Harper JI, Godwin H, Green A, Wilkes LE, Holden NJ, et al. A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis. Br J Dermatol. 2010 Feb 1, 162(2):397–403.
10. Del Carlo M, Schwartz D, Erickson EA, Loeser RF. Endogenous production of reactive oxygen species is required for stimulstion of human articular chondrocyte matrix metalloproteinase production by fibronectin fragments. Free Radical Biol Med. 2007, 42:1350–1358.
11. Sze S, Kleijn D, Lai R, Tan E, Zhao H, et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693
12. Chandrasekar B, Mummidi S, Mahimainathan L, Patel DN, Baile SR, et al. Interleukin-18-induced Human Coronary Artery Smooth Muscle Cell Migration is Dependent on NF-kB- and AP-1-mediated Matrix Metalloproteinase-9 Expression and is inhibited by Atorvastatin. J Biol Chem. 2006:281(22):15099-15109.
13. Cortez DM, Feldman MD, Mummidi S, Valente AJ, Steffensen B, et al. IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta, NF-kB, and AP-1 activation. Am J Physiol Heart Circ Physiol. 2007, 293:H3356–H3365.
14. Smith GN Jr, Mickler EA, Payne KK, Lee J, et al. Lung Transplant Metalloproteinase Levels are Elevated Prior to Bronciolitis Obliterans Syndrome. Am J Transplant. 2007, 7:1656–1661.
15. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
16. Patil P., Joshi M., Kuna Vijay., Xu B., Johannesson L., Olausson M., Sumitran-Holgersson S. CD271 identifies functional human hepatic stellate cells, which localize in peri-sinusoidal and portal areas in liver after partial hepatectomy. [Published same as previous]
17. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458
18. Zhu S., Hong J., Tripathi M., Sehdev V., et al. Regulation of CXCR4-Mediated Invasion by DARPP-32 in Gastric Cancer Cells. Mol Cancer Res January 2013 11, 86
19. Carduner L., Leroy-Dudal J., Picot CR., Gallet O., Carreiras F., et al. Ascites-induced shift along epithelial-mesenchymal spectrum in ovarian cancer cells: enhancement of their invasive behavior partly dependant on alphav integrins. Clinical and Experimental Metastasis. Jun 2014 Epub Ahead of Print.
20. Krohn A., et al. Tumor Cell Heterogeneity in Small Cell Lung Cancer (SCLC): Phenotypical and Functional Differences Associated with Epithelial-Mesenchymal Transition (EMT) and DNA Methylation Changes. PLOS One Published: June 24, 2014 DOI: 10.1371/journal.pone.0100249
21. Orgaz J., et al. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nature Communications 5, Article number:4255. doi:10.1038/ncomms5255
22. Sakimoto T., et al. Simultaneous study of matrix metalloproteinases, proinflammatory cytokines, and soluble cytokine receptors in the tears of noninfectious corneal ulcer patients. Graefes Arch Clin Exp Ophthalmol Pubslished online July 2014. DOI 10.1007/s00417-014-2708-1
23. Santosh N., et al. Matrix Metalloproteinase Expression During Blastema Formation in Regeneration-Competent Versus Regeneration-Deficient Amphibian Limbs. DEVELOPMENTAL DYNAMICS 240:1127–1141, 2011. DOI 10.1002/dvdy.22503
Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 829,59 €
Preis: 829,59 €
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