Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Array |
Specific against |
Rat |
Menge |
8 Sample Kit |
Host |
Hamster - Armenian |
ArtNr |
AAR-AKI-G1-8 |
Targets |
CCL2;;CST3;;FABP1;;HAVCR1;;LCN2;;TIMP1;;VEGFA |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Detection Method |
Fluorescence Laser Scanner |
Features |
- High sensitivity and specificity
- Low sample volume (10-100 ?l per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
|
Kit Components |
- Rat Acute Kidney Injury Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
|
Number of Targets Detected |
7 |
Other Materials Required |
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Result Output |
Semi-Quantitative |
Short description |
RayBio G-Series Rat Acute Kidney Injury Antibody Array 1 Kit. Detects 7 rat Cytokines. Suitable for all liquid sample types. 4 sample kit. |
Solid Support |
Glass Slide |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at ? -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL2, Cystatin C, FABP1, KIM-1, Lipocalin-2, MCP-1, NGAL, TIM-1, TIMP-1, VEGF-A |
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