Comparison

Rat AKI Array G1

Manufacturer Raybiotech
Category
Type Assay
Specific against Rat
Amount 8 Sample Kit
Item no. AAR-AKI-G1-8
Targets CCL2;;CST3;;FABP1;;HAVCR1;;LCN2;;TIMP1;;VEGFA
eClass 6.1 32161000
eClass 9.0 32161000
Available
Short description
RayBio G-Series Rat Acute Kidney Injury Antibody Array 1 Kit. Detects 7 rat Cytokines. Suitable for all liquid sample types. 4 sample kit.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ?l per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
7
Target Names
TIM-1 (KIM-1), FABP1, MCP-1 (CCL2), Cystatin C, Lipocalin-2 (NGAL), TIMP-1, VEGF-A
Targets Detected
CCL2, Cystatin C, FABP1, KIM-1, Lipocalin-2, MCP-1, NGAL, TIM-1, TIMP-1, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Rat Acute Kidney Injury Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at ? -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: Out of stock
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