Human BDNF ELISA

Hersteller Raybiotech
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Menge 1 x 96-Well Strip Microplate Kit
Host E.coli
ArtNr ELH-BDNF-1
Lieferbar
Quantity options
Short description
RayBio Human Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit for serum, plasma, cell culture supernatants, and urine
Target Name and Synonyms
BDNF
Solid Support
96-well Microplate
Design Principle
Sandwich-based
Result Output
Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Urine
Sensitivity
80 pg/ml
Detection Range
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma)
5-500 fold
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BLC, ENA-78, FGF-4, IL-1&alpha, , IL- 1&beta, , IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-&gamma, , Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1&alpha, , MIP-1 &beta, , MIP-1&delta, , PARC, PDGF, RANTES, SCF, TARC, TGF-&beta, , TIMP-1, TIMP-2, TNF-&alpha, , TNF-&beta, , TPO, VEGF).
Product Features
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Storage
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Citation
1. Browne AS., et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis. Fertil Steril. 2012 Sep, 98(3):713-9. doi: 10.1016/j.fertnstert.2012.05.027.
2. Uguz F, Sonmez EO, Sahingoz M, et al. Maternal generalized anxiety disorder during pregnancy and fetal brain development: A comparative study on cord blood brain-derived neurotrophic factor levels. J Psychosomatic Res. 2013 Aug 5, [Epub ahead of print] doi:10.1016/j.jpsychores.2013.07.002.
3. Oral E, Ozcan H, Kirkan TS, et al. Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder. Eur Arch Psychiatry Clin Neurosci. 2013 Mar 2, [Epub ahead of print] doi:10.1007/s00406-013-0398-z.
4. Browne A., Yu J., Huang R., et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis Fertility and Sterility Vol 98, Issue 3, September 2012, Pages 713–719
5. Liu Y., et al. Associations Between ApoEepsilon4 Carrier Status and Serum BDNF Levels—New Insights into the Molecular Mechanism of ApoEepsilon4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8 Actions in Alzheimer’s Disease
6. Sinkevicius K., et al. Neurotrophin receptor TrkB promotes lung adenocarcinoma metastasis. PNAS 2014 111 (28) 10299-10304, published ahead of print June 30, 2014, doi:10.1073/pnas.1404399111
7. Sahin S., et al. Effect of Methylphenidate Treatment on Appetite and Levels of Leptin, Ghrelin, Adiponectin, and Brain-Derived Neurotrophic Factor in Children and Adolescents with Attention Deficit and Hyperactivity Disorder. International Journal of Pyschiatry in Clinical Practice. Posted online on July 4, 2014. (doi:10.3109/13651501.2014.940054)
8. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
9. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883
Menge: 1 x 96-Well Strip Microplate Kit
Lieferbar: In stock
Listenpreis: 435,00 €
Rabatt: -15%
Preis: 369,75 €
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Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Sicherheitsdatenblatt

ELH-BDNF-1.pdf

Arbeitsanleitung

ELH-BDNF-1.pdf