Phospho-JNK (T183/Y185) and Total JNK ELISA Kit

Assay Kits|Phosphorylation Assays|ELISA Kits|Phosphorylation ELISA

-20°C

Sandwich-based

Semi-Quantitative

JNK1MAPK8PRKM8SAPK1SAPK1C

Distilled or deionized water 100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Mitogen-activated protein kinase 8 (MAP kinase 8) (MAPK 8) (EC 2.7.11.24) (JNK-46) (Stress-activated protein kinase 1c) (SAPK1c) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1)

The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185) and total (pan) JNK.

RayBio® Phospho- JNK (T183/Y185) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated JNK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-JNK. An anti-pan JNK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and JNK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-JNK (T183/Y185) antibody is used to detect phosphorylated JNK. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of JNK (T183/Y185) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

HepG2 cells were treated or untreated with 25ng/ml IL-1Î’ for 30 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.