Homocysteine Assay Kit

Homocysteine

Homocysteine (Hcy) Assay Kit is an assay kit for the detection and quantification of Homocysteine (Hcy) concentrations in serum, plasma and urine using a microplate reader. Oxidized homocysteine is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with the provided substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia further reacts with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

Not applicable.

Quantitative

Serum, plasma and urine.