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Anti-HA tag Antibody, Mouse Monoclonal

Anti-HA tag Antibody, Mouse Monoclonal

ArtNr	100028-MM10-5
Hersteller	Sino Biological
Menge	5 mL
Kategorie	Antibodies Flow Cytometry Immunoprecipitation Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Typ	Antibody Monoclonal
Applikationen	WB, FC, IF, IP, ICC, ELISA
Clon	10
Specific against	other
Host	Mouse
Isotype	IgG1
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	sino
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Antigen molecule name	HA
Storage	This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunogen	A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
Purification	Protein A
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Specificity	Recognize N-terminal and C-terminal HA Tag in fusion proteins.
Buffer	0.2 um filtered solution in PBS with 5% trehalose
Concentration	0.5 ul/Test
ELISA	This antibody can be used at 1/2000-1/5000 dilution with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).
Recommended WB Concentration	WB: 1/2000-1/5000
Recommended ELISA Concentration	ELISA: 1/2000-1/5000
Recommended ICC-IF Concentration	ICC/IF: 1/200-1/500
Recommended IP Concentration	IP: 2-8 uL/mg of lysate

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Menge: 5 mL

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