Recombinant Canine TNF-alpha Protein

ArtNr	70003-DNAE-1
Hersteller	Sino Biological
Menge	1 mg
Quantity options	1 mg 10 ug 100 ug 2 mg 4 mg 50 ug
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Typ	Proteins
Specific against	Dog (Canine, Canis lupus familiaris)
Host	E.coli
Purity	> 96 % as determined by SDS-PAGE
Sequence	Val77-Leu233
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	70003-DNAE
Similar products	TNF
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	Native
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TNF
Protein Construction	A DNA sequence encoding the soluble form of canine TNFalpha (NP_001003244.4) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Expressed Host	E.coli
Bio-Activity	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1.2 ng/ml.
Endotoxin	Please contact us for more information.
Predicted N Terminal	Met
Molecule Mass	The recombinant canine TNFalpha consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.
Formulation	Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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70003-DNAE-1-datasheet.pdf

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Menge: 1 mg

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