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Recombinant Canine TNF-alpha Protein

Recombinant Canine TNF-alpha Protein

Item no.	70003-DNAE-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Specific against	Dog (Canine, Canis lupus familiaris)
Host	E.coli
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	70003-DNAE
Similar products	TNF
Available
Manufacturer - Category	Protein
Molecule	TNF
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the soluble form of canine TNFalpha (NP_001003244.4) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Expressed Host	E.coli
Purity	> 96 % as determined by SDS-PAGE
Bio-Activity	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D . The ED50 for this effect is typically 0.2-1.2 ng/ml .
Endotoxin	Please contact us for more information.
Predicted N Terminal	Met
Molecule Mass	The recombinant canine TNFalpha consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.
Formulation	Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0
Accession	NP_001003244.4
Amino acid sequence	Val77-Leu233
TAG	Native

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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