Recombinant Human IGFBP-3 Protein (His Tag)

N-His

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2) (Gly28-Lys 291) was fused with a polyhistidine tag at the N-terminus.

1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 (Cat:13032-H24E) at 10 ug/mL (100 ul/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 (Cat:10598-H24E) at 10 ug/mL (100 ul/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL.
3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.02-0.2 ug/mL in the presence of 14 ng/mL human IGFII.

His 1

Lyophilized from sterile PBS, pH 7.4