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Recombinant Human FGFR1 Protein (His & GST Tag)

Recombinant Human FGFR1 Protein (His & GST Tag)

ArtNr	10616-H20B1-4
Hersteller	Sino Biological
Menge	4 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Astrocytoma By Organ Bladder Cancer
Typ	Proteins
Specific against	Human (Homo sapiens)
Konjugat/Tag	GST, HIS
Purity	> 93 % as determined by SDS-PAGE
Sequence	Gly311-Arg731
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	bFGF-R-1,BFGFR,CD331,CEK,FGFBR,FGFR-1,FLG,FLT-2,FLT2,HBGFR,HH2,HRTFDS,KAL2,N-SAM,OGD
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-GST & His
Shipping Temperature	Blue ice shipping
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	FGFR1
Protein Construction	A DNA sequence encoding the human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. Kinase activity untested 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF acidic (Cat:10013-HNAE) with a linear range of 15.6-250 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF basic (Cat:10014-HNAE) with a linear range of 0.16-2.5 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 75 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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10616-H20B1-4-datasheet.pdf

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Menge: 4 mg

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