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Human FGFR1 / CD331 Protein (His & GST Tag)

Human FGFR1 / CD331 Protein (His & GST Tag)

Hersteller	Sino Biological
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Astrocytoma By Organ Bladder Cancer
Typ	Proteins
Specific against	Human
Menge	20ug
ArtNr	10616-H20B1-20
eClass 6.1	34160400
eClass 9.0	42020190
Lieferbar
Molecule	FGFR1
Synonym	bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 93 % as determined by SDS-PAGE
Bio-Activity	1. Kinase activity untested 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF acidic (Cat:10013-HNAE) with a linear range of 15.6-250 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF basic (Cat:10014-HNAE) with a linear range of 0.16-2.5 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 75 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT
Accession	NP_075594.1
Amino acid sequence	Gly311-Arg731
TAG	N-GST & His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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10616-H20B1-20-datasheet.pdf

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Menge: 20ug

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