Human AMPK (G1/B1/A1) Heterotrimer Protein

Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Human
Format Baculovirus-Insect cells
Menge 20 ug
Host Baculovirus
ArtNr CT001-H0907B-20
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Molecule
AMPK
Synonym
5' adenosine monophosphate-activated protein kinas, 5' AMP-activated protein kinase, AMPK, beta 1 non-catalytic subunit and gamma 1 non-catal
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction
A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
Expressed Host
Baculovirus-Insect Cells
Purity
> 94 % as determined by SDS-PAGE
Bio-Activity
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met & Met & His
Molecule Mass
The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4
Accession
P54619 & Q9Y478 & Q13131-1
Amino acid sequence
Met1-Pro331 & Met1-Ile270 & Met1-Gln559
TAG
N-GST & N-His
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 375,97 €
Preis: 375,97 €
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