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Recombinant Human AMPK (G1/B1/A1) Heterotrimer Protein (His & GST Tag)

Recombinant Human AMPK (G1/B1/A1) Heterotrimer Protein (His & GST Tag)

ArtNr	CT001-H0907B-50
Hersteller	Sino Biological
Menge	50 ug
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Kategorie	Oncology Metabolism By Cell Type Hematology Lymphoma Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Status Autophagy
Typ	Proteins
Specific against	Human (Homo sapiens)
Konjugat/Tag	GST, HIS
Purity	> 94 % as determined by SDS-PAGE
Dry ice	Yes
Sequence	Met1-Pro331 & Met1-Ile270 & Met1-Gln559
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	5' adenosine monophosphate-activated protein kinas,5' AMP-activated protein kinase,AMPK,beta 1 non-catalytic subunit and gamma 1 non-catal
Versandbedingung	Trockeneis
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-GST & N-His
Shipping Temperature	Dry ice
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	AMPK
Protein Construction	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met & Met & His
Molecule Mass	The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 ug

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