AP12 (Apelin 12) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

Sample Type:
Serum, plasma and other biological fluids.

Intended Use:
Competitive ELISA, Coated with Antigen

Sensitivity:
<18.75pg/ml

Range:
31.25-2000pg/ml

Specificity:
Human

Test Principle:
This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AP12. During the reaction, AP12 in the sample or standard competes with a fixed amount of AP12 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AP12. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of AP12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Components:
Microtiter Strips, 1x48 wells or 1x96 wells
Standard, 1x1 vial or 2x1 vials
Sample/Standard Dilution Buffer, 1x10ml or 1x20ml
Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul
Antibody Dilution Buffer, 1x5ml or 1x10ml
Streptavidin (HRP) (SABC), 1x60ul or 1x120ul
SABC Dilution Buffer, 1x5ml or 1x10ml
TMB Substrate, 1x5ml or 1x10ml
Stop Solution, 1x5ml or 1x10ml
Wash Buffer, 25X, 1x15ml or 1x30ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.