NT-3, Recombinant, Human (Neurotrophin-3, Neurotrophic Gactor, Nerve Growth Factor-2, NGF-2, HGNF)

Blue Ice

27, 2

Supplied as a lyophilized powder from 20mM PBS, pH 7.2. Reconstitute with sterile PBS, 0.1% BSA to 0.1mg/ml.

NT-3 is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical.

Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (240 total amino acid residues). Human and mouse NT-3 sequences are identical.

Biological Activity:
The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50ng/ml.

AA Sequence:
MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

Biological Activity:
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.