Kidney Injury Molecule 1 (Kim1) Recombinant, Canine

Blue Ice

12, 6

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to Gln23-Glu125 with an N-Terminal His-Tag from canine Kim1 from E. coli (NM_001205114.1).

Amino Acid Sequence:
QVNGVVGH PATLPCTYST ASGVTTMCWG RGACPISHCL EEIVWTNGSH VTFQKHLRYK LKGKLSEGDV SLTIENAAQT DSGQYCCRVE HRGWFNDMKL TLSLE

Predicted Molecular Weight:
12.6kD

Predicted Isoelectric Point:
6.8

Accurate Molecular Mass:
14kD as determined by SDS-PAGE reducing conditions

Tissue Specificity:
Membrane

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS, pH 7.4. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Included Protein Molecular Weight Marker:
168631: Unstained Protein Molecular Weight Marker, 10-70kD
Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.

Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.

Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:
Mini gels: 3-5ul/well
Large gel: 7ul/well
The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.
Store at -20°C Stable for 6 months after receipt.