Plasminogen Activator, Urokinase Receptor (uPAR) BioAssay™ ELISA Kit (Rat)

Kits and Assays / Kits-ELISA (Receptor Proteins), BioAssay™

4°C/-20°C

Plasminogen Activator, Urokinase Receptor (uPAR) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of uPAR in rat serum, plasma and other biological fluids.

Detection Range:
0.312-20ng/ml

Sensitivity:
0.127ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*027490A: Microtiter Strips, 1x96 wells
*027490B: Standard, 2x1 vial
027490C: Standard Diluent, 1x20ml
*027490D: Detection Reagent A (green), 1x120ul
*027490E: Detection Reagent B (red), 1x120ul
027490F: Assay Diluent A, 1x12ml
027490G: Assay Diluent B, 1x12ml
027490H: TMB Substrate, 1x9ml
*027490J: Positive Control, 1.25ng/ml, 1x1 vial
027490K: Stop Solution, 1x6ml
027490L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (027490K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *027490A, *027490B, *027490D, *027490E and *027490J at -20°C. After reconstitution of *027490J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 027490J: Positive Control

Intended Use:
The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics:
The positive control vial contains rat uPAR in rat serum (lyophilized powder). Note: The rat based material used in this product is from healthy rats of SPF standard (Specific Pathogen-Free animals).

Expected Values:
1.25ng/ml

Usage:
Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.