Phenylalanine Hydroxylase (PAH) Recombinant, Human

Blue Ice

26, 9

Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose, . Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.

Recombinant protein corresponding to aa228-452 with an N-Ternminal His-Tag from human PAH, expressed in E. coli. Uniprot/Accession: P00439

Predicted Isoelectric Point:
6.1

Predicted Molecular Mass:
26.7kD

Accurate Molecular Mass;
26kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Evtracellular matrix

Amino Acid Sequence:
EDV SQFLQTCTGF RLRPVAGLLS SRDFLGGLAF RVFHCTQYIR HGSKPMYTPE PDICHELLGH VPLFSDRSFA QFSQEIGLAS LGAPDEYIEK LATIYWFTVE FGLCKQGDSI KAYGAGLLSS FGELQYCLSE KPKLLPLELE KTAIQNYTVT EFQPLYYVAE SFNDAKEKVR NFAATIPRPF SVRYDPYTQR IEVLDNTQQL KILADSINSE IGILCSALQK IK

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.