3-Methyladenine

Blue Ice

150, 95

Supplied as a white solid

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50=4.5mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 and Wortmannin.

Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis following serum/potassium deprivation.

Synonyms:
3-Methyl-3H-purin-6-amine; 3-Methyl-6-aminopurine; 3-Methyladenine; 6-Amino-3-methylpurine; N3-Methyladenine; NSC 66389

CAS Number:
5142-23-4

Molecular Formula:
C6H7N5•0.1H2O

Molecular Weight:
150.95

Purity:
≥99%

Appearance:
Supplied as a white solid

Solubility:
Soluble in DMSO to 20mM

Melting Point:
>260°C (dec.) (lit)

Method for Determining Identity:
Proton NMR Spectroscopic and Mass Spectrometric Analysis

Elemental Analysis:
Calculated: %C: 47.74, %H: 4.81, %N: 46.39

Storage and Stability:
Long-term storage is recommended at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Toxicity and Hazards: 
All products should be handled by qualified personnel only, trained in laboratory procedures.