IL-8 (Interleukin 8) BioAssay™ ELISA Kit (Mouse)

Kits and Assays / Kits-ELISA (Cytokines, Interleukins), BioAssay™

4°C/-20°C

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Intended Use:
Sandwich ELISA, Double Antibody

Sensitivity:
<9.375pg/ml

Range:
15.625-1000pg/ml

Specificity:
Mouse

Test Principle:
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 can be calculated.

Kit Components:
Microtiter Strips, 1x48 wells or 1x96 wells
Standard, 1x1 vial or 2x1 vials
Sample/Standard Dilution Buffer, 1x10ml or 1x20ml
Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul
Antibody Dilution Buffer, 1x5ml or 1x10ml
Streptavidin (HRP) (SABC), 1x60ul or 1x120ul
SABC Dilution Buffer, 1x5ml or 1x10ml
TMB Substrate, 1x5ml or 1x10ml
Stop Solution, 1x5ml or 1x10ml
Wash Buffer, 25X, 1x15ml or 1x30ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.