Glucose Assay Kit is an Assay Kit by HK Method.
This product is used to determine glucose content in human serum.
Hexokinase catalyzes the reaction between glucose in the human serum and ATP to generate glucose - 6 - phosphate (G - 6 - P) and ADP. The G - 6 - P is oxidized by glucose - 6 - phosphate dehydrogenase (G - 6 - PD H) and forms 6 - p hosphogluconic acid (6 - GP), at the same time, reduction re action is occurred to transform NAD+ to NADH. The content of NADH is proportional to the glucose concentration. The glucose concentration in the serum is calculated by detecting the increase value of NADH absorbance at 340nm.
R1: Detection Reagent 1 |
Storage |
Stored for up to 12 months at 2 - 8C, protect from light. After opening, the reagent remains stable for 30 days at 2 - 8C , protect from light. |
Buffer |
Not applicable. |
Test Range |
0.03 mmol/l - 40.00 mmol/l |
Dry Ice |
No |
Note |
This product is for research use only. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.