DNA Polymerase for PAGE Enzyme

Proteins and Peptides

Bacteria

DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. DNA Polymerase for PAGE has 5?-3? DNA polymerase activity and 5?-3? exonuclease activity. The extension rate is about 1-2 kb/min. This enzyme is supplied with a unique buffer compatible with PAGE, and the resulting PCR product is suitable for use in SDS-PAGE and agarose gel electrophoresis. The enzyme can amplify genomic DNA fragments up to 3 kb. This kit does not contain 2.5 mM dNTPs.Contents:

Component
Dry Ice	No
Buffer	DNA Polymerase for PAGE: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. 10X Buffer for PAGE: Contains 200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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