Human Apolipoprotein C3 (APOC3) Protein

Human Apolipoprotein C3 Protein is a recombinant Human protein expressed in E. coli.

This protein is the immunogen for the following antibodies: abx128596

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.

Calculated MW: 38.8 kDa
Observed MW (SDS-PAGE): 34 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Human

Apolipoprotein C3 (APOC3)