Comparison

Ni-NTA Resin European Partner

Item no. abx098108-5
Manufacturer Abbexa
Amount 5 ml
Quantity options 25 ml 5 ml
Category
Type Reagents
Specific against other
ECLASS 10.1 42051003
ECLASS 11.0 42051003
UNSPSC 12000000
Similar products Ni-NTA Resin
Available
Manufacturer - Category
Reagents and Tools
Manufacturer - Targets
Ni-NTA Resin
Storage Conditions
Store at 2-8 °C (with 20% ethanol) for up to 2 years.
Description
Ni-NTA Resin allows rapid affinity purification of His-tagged proteins. His-tagged proteins bind to Ni2+ cations, which are immobilized on the Ni-NTA resin by 4 metal-chelating sites. After unbound proteins are washed away, the target proteins are recovered by gradient elution. It is suitable for both native and denatured protein purification.Specifications:
  • Resin: Cross-linked 6% agarose
  • Ligand: NTA
  • Shape: Sphere
  • Pore Size: 45-165 µm
  • Binding Capacity: 10-20 mg/ml wet gel
  • Recommended Flow Rate: < 300 cm/h
  • Highest Resisistance of Atmospheric Pressure: 0.3 MPa
  • pH Stability: 3-13
Dry Ice
No
Buffer
Note: Buffers are not included with this product.

Equilibration Buffer for soluble proteins: 50 mM sodium phosphate buffer, 300 mM NaCl, 10 mM imidazole, 10 mM Tris-HCl, pH 8.0.
Equilibration Buffer for inclusion bodies: 100 mM sodium phosphate buffer, 6 M GuHCl, 10 mM Tris-HCl, pH 8.0; or 100 mM sodium phosphate buffer, 8 M urea, 10 mM Tris-HCl, pH 8.0.
Note
This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 ml
Available: In stock
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