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Bovine Peptidyl-prolyl cis-trans isomerase D (PPID) ELISA Kit

Bovine Peptidyl-prolyl cis-trans isomerase D (PPID) ELISA Kit

Item no.	ABK-KTE10198-96Tx50
Manufacturer	Abbkine Scientific
Amount	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Cattle (Bovine)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PPID,CYP-40,CYPD,MGC33096,40 kDa peptidyl-prolyl cis-trans isomerase D,PPIase,cyclophilin 40,cyclophilin D,cyclophilin-related protein,rotamase
Available
Manufacturer - Applications	This Bovine Peptidyl-prolyl cis-trans isomerase D (PPID) ELISA Kit employs a two-site sandwich ELISA to quantitate PPID in samples. An antibody specific for PPID has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPID present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPID is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPID bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Cyclophilin D, which is located in the matrix of mitochondria, is a component of the mitochondrial permeability transition pore. The pore opening raises the permeability of the mitochondrial inner membrane, allows influx of cytosolic molecules into the mitochondrial matrix, increases the matrix volume, and disrupts the mitochondrial outer membrane. As a result, the mitochondria fall into a functional disorder, so the opening of the pore plays an important role in cell death. Cyclophilin D is thought to regulate the opening of the pore because cyclosporin, which binds to CyP-D, inhibits the pore opening. Cyclophilin-40 (CyP40, also CYPD) was identified by Kieffer et al. (1992) as a 40-kD cyclophilin-like protein with PPIase activity. In the bovine uterus, CyP40 is a component of the estrogen receptor complex.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Bovine Peptidyl-prolyl cis-trans isomerase D (PPID) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PPID. No significant cross-reactivity or interference between Bovine PPID and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Bovine Peptidyl-prolyl cis-trans isomerase D microplate Bovine Peptidyl-prolyl cis-trans isomerase D standard Bovine Peptidyl-prolyl cis-trans isomerase D detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T x 50

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Delivery expected until 11/13/2025

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