Bovine Secretory phospholipase A2 receptor (PLA2R1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Bovine Secretory phospholipase A2 receptor microplate
Bovine Secretory phospholipase A2 receptor standard
Bovine Secretory phospholipase A2 receptor detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Sequence analysis revealed that the deduced 1, 465-amino acid PLA2R1 transmembrane protein contains a 25-amino acid signal sequence, 15 potential N-glycosylation sites, an N-terminal cysteine-rich domain, a fibronectin-like type II domain, 8 carbohydrate recognition domains, and a short C-terminal cytoplasmic tail containing a consensus sequence (asn-pro-X-tyr) also found in bovine Pla2r1. A casein-kinase II phosphorylation site is also located in this C-terminal region. Ancian et al. (1995) identified a splice variant encoding a soluble form of PLA2R1. Quantitative PCR analysis suggested that the ratio for the transcription of membrane to soluble PLA2R1 is 1.6. Northern blot analysis detected strong expression of 6.5- and 5.4-kb PLA2R1 transcripts in kidney, with moderate expression in placenta, lung, and skeletal muscle.

PLA2R1; CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R; phospholipase A2 receptor 1