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Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit

Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit

Item no.	ABK-KTE10231-96Tx50
Manufacturer	Abbkine Scientific
Amount	96Tx50
Category	Oncology Neuroscience Neural Lineage Markers Cell Biology Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Bovine
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Applications notes	This Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit employs a two-site sandwich ELISA to quantitate MYLK in samples. An antibody specific for MYLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Bovine Myosin light chain kinase, smooth muscle microplate Bovine Myosin light chain kinase, smooth muscle standard Bovine Myosin light chain kinase, smooth muscle detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Bovine Myosin light chain kinase, smooth muscle (MYLK) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MYLK. No significant cross-reactivity or interference between Bovine MYLK and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Myosin light chain kinase, smooth muscle (MYLK), a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. MYLK encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
Gene ID	338037
Alternative	MYLK; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; OTTHUMP00000180642; myosin; light polypeptide kinase; smooth muscle myosin light chain kinase
Accession	Q28824

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96Tx50

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Delivery expected until 9/18/2025

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